Pseudo-UTP (100 mM)

 

    Pseudouridine, discovered in 1951, is the first identified modified nucleoside in RNA and is considered the "fifth nucleoside" in RNA. It is widely present in tRNA, mRNA, snRNA, and snoRNA, and has extensive applications in nucleic acid therapeutics. Research by the Karikó group at the University of  Pennsylvania has shown that replacing uridine with pseudouridine can significantly reduce the immunogenicity of in vitro transcribed mRNA, improve mRNA stability, and increase expression. This colorless and transparent liquid is primarily used in oligonucleotide aptamer, epigenetics or DNA damage research, in vitro transcription (IVT), mutagenesis induction, and photoreactive crosslinking studies.