Bst II DNA Polymerase (Large Fragment)
REF : EG23101
REF No.: | EG23101S | EG23101M |
Specs: | 1600 U | 8000 U |
Price: | $96 | $420 |
Description
Bst II DNA Polymerase (Large Fragment) is a modified form of Bst DNA polymerase obtained by truncating the 5' → 3' exonuclease domain. Through directed evolution and recombinant expression in Escherichia coli, this enzyme has been engineered. It possesses robust 5' → 3' DNA polymerase activity and strand displacement activity. The optimal reaction temperature for this enzyme is 65°C, and it is suitable for various isothermal amplification reactions.Compared to the wild-type enzyme, Bst II DNA Polymerase (Large Fragment) exhibits faster amplification rates, enhanced tolerance to dUTP and salt, as well as improved stability.
Components
Component | EG23101S | EG23101M |
Bst II DNA Polymerase (Large Fragment) (8 U/μl) | 200 μl | 1 ml |
10× Bst II Reaction Buffer | 1 ml | 3×1 ml |
MgSO4 (100 mM) | 1 ml | 3×1 ml |
Storage Condition
-20