Nb.BsrDI
REF : EG23514S
| Specs： | 2000 U |
| Price： | $158 |
| Cut site： | GCAATGNN |
| 5'...G C A A T G N N...3' 3'...C G T T A C↑N N ...5' |
Description
Nb.BsrDI is a nicking endonuclease that cuts only one strand of the dsDNA substrate; it introduces a nick into the dsDNA substrate without cleaving the dsDNA.
Components
| Component | Amount |
| Nb.BsrDI (10 U/μl) | 200 μl |
| 10× CutOne™ Buffer | 2×1 ml |
Features and Usage
Recommended Reaction Conditions
1× CutOne™ Buffer;
Incubate at 65℃ ;
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 50% activity when performing enzymatic digestion reactions at 37℃ .
Incubate at 65℃ ;
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 50% activity when performing enzymatic digestion reactions at 37℃ .
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Storage Condition
-20