Nt.BspQI
REF : EG23512S
| Specs： | 2000 U |
| Price： | $158 |
| Cut site： | GCTCTTCN |
| 5'...G C T C T T C N↓...3' 3'...C G A G A A G N ...5' |
Description
Nt.BspQI is a nicking endonuclease that cuts only one strand of the dsDNA substrate; it introduces a nick into the dsDNA substrate without cleaving the dsDNA.
Components
| Component | Amount |
| Nt.BspQI (10 U/μl) | 200 μl |
| 10× Cut Buffer C | 2×1 ml |
Features and Usage
Recommended Reaction Conditions
1× Cut Buffer C；
Incubate at 50℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 75% activity when performing enzymatic digestion reactions at 37℃ .
Incubate at 50℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 75% activity when performing enzymatic digestion reactions at 37℃ .
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Storage Condition
-20