M-MLV GIII Reverse Transcriptase
REF : EG15124S
Specs: | 10000 U |
Price: | $50 |
Description
M-MLV GIII Reverse Transcriptase is third-generation reverse transcriptase derived through genetic engineering and recombinant technology from the Moloney Murine Leukemia Virus (M-MLV). This enzyme has been modified to eliminate the RNase H activity. Additionally, it exhibits significantly improved thermal stability(optimal reaction temperature:50 ℃). These greatly improve the specificity and length of the synthesized first-strand cDNA, allowing for the synthesis of cDNA fragments up to 12 kb. Moreover, M-MLV GIII Reverse Transcriptase shows enhanced tolerance to RNA secondary structures.
Components
Component |
Amount |
M-MLV GIII Reverse Transcriptase(200 U/μl) |
50 μl |
5× M-MLV First Strand Buffer |
500 μl |
0.1 M DTT |
100 μl |
Storage Condition
-20℃